Darcie L. Moore

Position title: Associate Professor || PhD - Neuroscience, University of Miami, Florida || Post-doc - ETH Zürich/University of Zürich, Switzerland

Email: darcie.moore@wisc.edu

Phone: Phone: (608) 265-7836 | Fax: (608)265-5512

Address:
RESEARCH INTERESTS - Neural stem cells and aging

Darcie Moore

Moore Lab Website

Positions available:
Graduate students
Postdoctoral fellows

We are a lab that gets excited about science.

If you do too, please contact Dr. Moore for more information on specific projects.

There is limited regenerative capacity in the adult mammalian central nervous system (CNS). My lab studies this loss of regeneration in two different contexts:

Adult neurogenesis, where neural stem cells (NSCs) make newborn neurons in the adult brain

CNS axon regeneration following an injury

Specifically we determine how changes during development and aging influence these aspects of regeneration.

Adult neurogenesis

NSCs in the hippocampus generate newborn neurons throughout life in a process referred to as adult neurogenesis. Adult NSCs are primarily quiescent, in a reversible G0 state. Upon receiving a signal, quiescent NSCs (qNSCs) activate, entering the cell cycle to initiate population expansion, differentiation, maturation, and integration. During aging and disease, extrinsic and intrinsic factors drive adult hippocampal NSCs deeper into quiescence, reducing NSC quiescence exit, ultimately contributing to cognitive decline. My lab works to identify factors controlling NSC quiescence and quiescence exit to improve neurogenesis and ultimately identify targets to enhance cognitive function. We specifically have focused our efforts in the following areas: Proteostasis, translational control, asymmetric inheritance of specific cellular cargoes during mitosis, creation of imaging tools to identify qNSCs, how this translates to cancer.

CNS Axon Growth and Regeneration

Despite decades of study in rodent models, there is still no “cure” for CNS injuries such as spinal cord injury (SCI). The lack of human-specific and age-specific models of axon growth may be a crucial limiting factor in identifying translational targets for treatment of human SCI. Thus, we used direct reprogramming of human fibroblasts to neurons, bypassing pluripotency to maintain the age of the original cell, to create human neurons of different ages. We hypothesize that a species- and age-specific model will lead to the identification of axon growth regulators that could be targeted for development of more effective therapies for human SCI. Our research in this area has focused on: the developmental loss of intrinsic axon growth ability in human neurons, epigenetic regulation of axon growth and regeneration.

To address these questions, the Moore lab uses cell biology, biochemistry, molecular biology, genetics, and computational approaches. We also specifically focus on using advanced live imaging technologies, including FLIP, FLIM, FRAP, photoactivation, 4D timelapse, in vivo cranial window imaging, and computer learning-based high-throughput imaging to address our scientific questions. If you can see it, you can believe it.


Selected Recent Honors:

2022 – Vilas Associate, UW-Madison

2021 – “The Ride” Scholar, UW-Madison Carbone Cancer Center

2020 – Vallee Scholar Award, Vallee Foundation

2019 – Shaw Scientist Award, Greater Milwaukee Foundation

2019 – “Rising Star in Neuroscience” The Scientist magazine

2018 – NIH Director’s New Innovator Award (DP2)

2017 – Sloan Research Fellowship, Alfred P. Sloan Foundation

2016 – Peter and Patricia Gruber International Research Award, Society for Neuroscience

Selected Publications:

  • Morrow CS, Tweed K, Farhadova S, Walsh AJ, Lear BP, Roopra A, Risgaard RD, Klosa PC, Arndt ZP, Peterson ER, Chi MM, Harris AG, Skala MC, Moore DL (2024). Autofluorescence is a biomarker of neural stem cell activation state. Cell Stem Cell, accepted.
  • Lear BP, Moore DL (2023). Moving CNS axon growth and regeneration research into human model systems, Frontiers in Neuroscience. 17:1198041. PMID: 37425013
  • Lear BP, Thompson EAN, Rodriguez K, Arndt ZP, Khullar S, Klosa PC, Lu RJ, Morrow CS, Peterson ER, Teefy BB, Bhattacharyya A, Sousa AMM, Wang D, Benayoun B, Moore DL (2023). Age-maintained human neurons demonstrate a developmental loss of intrinsic neurite growth ability, bioRxiv, doi: 10.1101/2023.05.23.541995.
  • Morrow CS, Arndt ZP, Klosa PC, Peng B, Zewdie EY, Benayoun, BA, Moore DL (2022). Adult fibroblasts use aggresomes only in distinct cell-states. Scientific Reports Sep 2;12(1):15001. Pubmed PMID: 36056070; Pubmed Central PMCID: PMC9440096
  • M.K. Imtiaz, B. Jaeger, S. Bottes, R. Machado, M. Vidmar, D.L. Moore*, S. Jessberger* (2021). Declining Lamin B1 expression mediates age-dependent decreases of hippocampal stem cell activity. Cell Stem Cell, 28(5):967-977. PMID: 33631115 *co-corresponding authors
  • Morrow CS, Porter TJ, Xu N, Arndt ZP, Ako-Asare K, Heo HJ, Thompson EAN, Moore DL. (2020). Vimentin Coordinates Protein Turnover at the Aggresome during Neural Stem Cell Quiescence Exit. Cell Stem Cell, Apr 2;26(4):558-568.e9. Pubmed PMID: 32109376; Pubmed Central PMCID: PMC7127969.
  • C.S. Morrow, D.L. Moore (2020). Vimentin’s side gig: Regulating cellular proteostasis in mammalian systems. Cytoskeleton, 77(11): 515-523. PMID: 33190414; PubMed Central PMCID: PMC8216102.

For additional publications, please click here.