Department of Neuroscience
School of Medicine and Public Health

Jon Audhya

Position title: Professor, Department of Biomolecular Chemistry

Email: audhya@wisc.edu

Phone: (608) 262-3761

Address:
RESEARCH INTERESTS - Understanding the fundamental mechanisms by which membrane proteins, lipids, and other macro molecules are transported throughout eukaryotic cells.

Jon Audhya

Audhya Lab Website

5214 Biochemical Sciences Building

440 Henry Mall Madison, WI 53706

Education

  • B.S. 1997, Brown University, Providence, RI
  • Ph.D. 2002, University of California, San Diego (S. Emr)
  • Postdoctoral 2003-07, Ludwig Institute for Cancer Research (K. Oegema)

Honors & Awards

  • Sigma Xi Honorary Society, 1997
  • Bachelor of Science, Magna cum Laude, Brown University, 1997
  • Harvey Almy Baker Graduate Fellowship, Brown University, 1997
  • National Cancer Institute Training Grant Award, 1998-2002
  • Helen Hay Whitney Foundation Post-Doctoral Fellowship, 2004-2007
  • DeLill Nasser Award for Professional Development in Genetics, 2006
  • March of Dimes Basil O’Connor Starter Scholar Research Award, 2010
  • American Heart Association Scientist Development Grant Award, 2010
  • Shaw Scientist Award, 2010
  • American Cancer Society Research Scholar Award, 2012
  •  Vilas Associate Award, University of Wisconsin-Madison, 2015
  •  H.I. Romnes Faculty Fellowship, UW Graduate School/WARF, 2017

Research Interests

Our laboratory is committed to understanding fundamental mechanisms by which membrane proteins, lipids, and other macromolecules are transported throughout eukaryotic cells. To do so, we take advantage of numerous interdisciplinary approaches, including biochemistry, structural biology, biophysics, genetics, molecular biology and high resolution fluorescence and electron microscopy.

Additionally, we use a variety of experimental systems, ranging from simple animal models (e.g. Caenorhabditis elegans) to human induced pluripotent stem cells (iPSCs). We also aim to recapitulate individual steps of membrane transport in vitro, using recombinant proteins and chemically defined lipids. Our ultimate goal is to identify the regulatory pathways that control membrane deformation, which enable vesicle formation in the endosomal and secretory systems. Although basic research is the cornerstone of our program, we also seek to define pathomechanisms that underlie human disease, focusing on the impact of mutations in key trafficking components that lead to cancer, neurodegeneration, asthma, and diabetes. For more information, please visit our lab webpage: www.audhyalab.org.

Publications of Note

Preform a customized  PubMed literature search for Dr. Audhya.

  • Johnson A, Bhattacharya N, Hanna M, Pennington JG, Schuh AL, Wang L, Otegui MS, Stagg SM, Audhya A (2015) TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization. EMBO J. In Press.
  • Takahashi H, Mayers JR, Wang L, Edwardson JM, Audhya A (2015) Hrs and STAM function synergistically to bind ubiquitin-modified cargoes in vitro. Biophys. J. 108(1):76-84.
  • Schuh AL, Hanna M, Quinney K, Wang L, Sarkeshik A, Yates III JR, Audhya A (2015) The VPS-20 Subunit of the Endosomal Sorting Complex ESCRT-III Exhibits an Open Conformation in the Absence of Upstream Activation. Biochem. J. 466:625-637.
  • Hsu KT, Yu XM, Audhya AW, Jaume JC, Lloyd RV, Miyamoto S, Prolla TA, Chen H (2014) Novel approaches in anaplastic thyroid cancer therapy. Oncologist 19(11):1148-55.
  • Wan J, Zhu F, Zasadil LM, Yu J, Wang L, Johnson A, Berthier E, Beebe DJ, Audhya A, Weaver BA (2014) A Golgi-localized pool of the mitotic checkpoint component Mad1 controls integrin secretion and cell migration. Curr. Biol. 24(22):2687-92.
  • Kebede MA, Oler AT, Gregg T, Balloon AJ, Johnson A, Mitok K, Rabaglia M, Schueler K, Stapleton D, Thorstenson C, Wrighton L, Floyd BJ, Richards O, Raines S, Eliceiri K, Seidah NG, Rhodes C, Keller MP, Coon JL, Audhya A, Attie AD (2014) SORCS1 is necessary for normal insulin secretory granule biogenesis in metabolically stressed β cells. J. Clin. Invest. 124(10):4240-56.
  • Shen QT, Schuh AL, Zheng Y, Quinney K, Wang L, Hanna M, Mitchell JC, Otegui MS, Ahlquist P, Cui Q, Audhya A (2014) Structural analysis and modeling reveals new mechanisms governing ESCRT-III spiral filament assembly. J. Cell Biol. 206(6):763-77.
  • Wang L, Audhya A (2014) In vivo imaging of C. elegans endocytosis. Methods 68(3):518-28.
  • Schuh AL, Audhya A (2014) The ESCRT machinery: from the plasma membrane to endosomes and back again. Crit. Rev. Biochem. Mol. Biol. 49(3):242-61.
  • Wickert LE, Karta MR, Audhya A, Gern JE, Bertics PJ (2014) Simvastatin attenuates rhinovirus-induced interferon and CXCL10 secretion from monocytic cells in vitro. J. Leukoc. Biol. 95(6):951-9.
  • Bahmanyar S, Biggs R, Schuh AL, Desai A, Müller-Reichert T, Audhya A, Dixon JE, Oegema K (2014) Spatial control of phospholipid flux restricts endoplasmic reticulum sheet formation to allow nuclear envelope breakdown. Genes Dev. 28(2):121-6.
  • Green RA, Mayers JR, Wang S, Lewellyn L, Desai A, Audhya A, Oegema K (2013) The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules. J. Cell Biol. 203(3):505-20.
  • Mayers JR, Wang L, Pramanik J, Johnson A, Sarkeshik A, Wang Y, Saengsawang W, Yates JR, Audhya A (2013) Regulation of ubiquitin-dependent cargo sorting by multiple endocytic adaptors at the plasma membrane. Proc. Natl. Acad. Sci. U.S.A. 110(29):11857-62.
  • Hanna M, Wang L, Audhya A (2013) Worming our way in and out of the Caenorhabditis elegans germline and developing embryo. Traffic 14(5):471-8.
  • Morrison K, Witte K, Mayers JR, Schuh AL, Audhya A (2012) Roles of acidic phospholipids and nucleotides in regulating membrane binding and activity of a calcium-independent phospholipase A2 isoform. J. Biol. Chem. 287(46):38824-34.
  • Ryan SD, Britigan EM, Zasadil LM, Witte K, Audhya A, Roopra A, Weaver BA (2012) Up-regulation of the mitotic checkpoint component Mad1 causes chromosomal instability and resistance to microtubule poisons. Proc. Natl. Acad. Sci. U.S.A. 109(33):E2205-14.
  • Schuh AL, Audhya A (2012) Phosphoinositide signaling during membrane transport in Saccharomyces cerevisiae. Subcell. Biochem. 59:35-63.
  • Mayers JR, Audhya A (2012) Vesicle formation within endosomes: An ESCRT marks the spot. Commun Integr Biol 5(1):50-6.
  • Fyfe I, Schuh AL, Edwardson JM, Audhya A (2011) Association of the endosomal sorting complex ESCRT-II with the Vps20 subunit of ESCRT-III generates a curvature-sensitive complex capable of nucleating ESCRT-III filaments. J. Biol. Chem. 286(39):34262-70.
  • Park S, Patterson EE, Cobb J, Audhya A, Gartenberg MR, Fox CA (2011) Palmitoylation controls the dynamics of budding-yeast heterochromatin via the telomere-binding protein Rif1. Proc. Natl. Acad. Sci. U.S.A. 108(35):14572-7.
  • Green RA, Kao HL, Audhya A, Arur S, Mayers JR, Fridolfsson HN, Schulman M, Schloissnig S, Niessen S, Laband K, Wang S, Starr DA, Hyman AA, Schedl T, Desai A, Piano F, Gunsalus KC, Oegema K (2011) A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue. Cell 145(3):470-82.
  • Witte K, Schuh AL, Hegermann J, Sarkeshik A, Mayers JR, Schwarze K, Yates JR, Eimer S, Audhya A (2011) TFG-1 function in protein secretion and oncogenesis. Nat. Cell Biol. 13(5):550-8.
  • Mayers JR, Fyfe I, Schuh AL, Chapman ER, Edwardson JM, Audhya A (2011) ESCRT-0 assembles as a heterotetrameric complex on membranes and binds multiple ubiquitinylated cargoes simultaneously. J. Biol. Chem. 286(11):9636-45.